Unit 3: Gene Cloning - Biotechnology Pdf.

Unit 3: Gene Cloning - Table of Contents

Topic
Gene Cloning
Recombinant DNA
Bacterial Transformation and Selection of Recombinant Clones
PCR-mediated Gene Cloning
Gene Construct
Construction of Genomic and cDNA Libraries
Screening DNA Libraries to Obtain Gene of Interest by Genetic Selection
Complementation
Colony Hybridization
PCR
Methods of Gene Transfer
Agrobacterium-mediated
Direct Gene Transfer by Electroporation
Microinjection
Microprojectile Bombardment
Selection of Transgenics – Selectable Marker and Reporter Genes (Luciferase, GUS, GFP)

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